TEST FOR SUGAR USING BENEDICT's SOLUTION
Benedict's reagent (also called Benedict's solution or Benedict's test) is a chemical reagent named after an American chemist, Stanley Rossiter Benedict.
Benedict's reagent is used as a test for the presence of reducing sugars. This includes allmonosaccharides and the disaccharides, lactose and maltose. Even more generally, Benedict's test will detect the presence of aldehydes (except aromatic ones), and alpha-hydroxy-ketones, including those that occur in certain ketoses. Thus, although the ketose fructose is not strictly a reducing sugar, it is an alpha-hydroxy-ketone, and gives a positive test because it is converted to the aldoses glucose and mannose by the base in the reagent.
Experiment | Observation | Inference |
---|---|---|
Substance in water + 3ml Benedict's solution, then boil for few minutes and allow to cool. | Red ppt or green ppt or yellow ppt obtained | Reducing sugar e.g. Glucose is present |
Substance in water + 3ml Benedict's solution, then boil for few minutes and allow to cool. | Solution remains clear | Reducing sugar is not present |

from left: haziq, oren, sabiq, rashid, ammar
group C3
thank to Benedict